run out of solvent with agilent system for 5 hours!

[vivian]

Hi,
I know it's a stupid mistake. Can anyone tell me what I should do to rescue the column? It's an SEC column. I am washing it wth water for 24 hours with 0.1 ml/min flow rate now. Will it affect the future analysis? Since the agilent system can auto-degas, I am hoping it will reduce the damage to the system and the column. Can anyone tell me how serious the situation is? Thanks!

[tom jupille]

There seems to be an epidemic of that going around. That topic was just covered a few days ago:

http://www.sepsci.com/chromforum/viewtopic.php?t=937

If it's any comfort, we've probably all done it at one time or another!