SEC in de-ionized water

[kishoreravuri]

While running SEC in just water, I found good RT and peaks for my sample. Is it fine to run SEC in just water? What standards can I use with just water as mobile phase( most that I know of are run in buffered systems)

[danko]

What kind of analyte/compound are you dealing with?
What about the matrix – if any?

Best Regards

[Uwe Neue]

Polyethyleneoxide standards are fine in straight water.

[kishoreravuri]

am dealing with nonionic surfactants

[danko]

Then Uwe’s suggestion is no less than perfect.
I was thinking of proteins. In such a case, some buffering would have been advisable.

Best Regrds