Chromatography Forum

Hi,
I have to analyze fosfomycin without having available LC-MS, GC or CE
I found a HPLC method which involves indirekt UV detection with a mobile phase containing acridine. however, I am afraid, that this stuff will be hard to get out of the system afterwards.

Now I thought of the following: is it possible to open the epoxide ring of fosfomycin - maybe by an amine or in a two-step reaction - to form an amino alcohol (which could then probably be detected with my good old amperometric detector or after derivatization)?

Please give some advice. Thank you!
Friederike

I don't think it will be too hard to wash acridine from a reverse phase column; however, I don't think your sensitivity will be good with this method.

Your idea of opening the epoxide ring with an amine sounds good as long as you can get good conversion to the redox active product.