Purity of standard

[ta068]

I have extracted a compound from nature product and I want to use this compound as standard for developing a analytical method using HPLC.

The question is how could I know the exact purity of this compound if it does not has any commercial working or reference standard.

[HW Mueller]

You will never get an exact purity, but you can probably get close enough for your purpose via NMR, MS, UV, IR, and elemental (carbon, hydrogen , , , ) analysis. Two very different chromatographic methods may also help, sometimes a sharp (constant after several recrystallizations) melting point is quite instructive.

[JGK]

Moisture content would also be helpful.