Silver Ion HPLC for Oil & Fat Analysis Using ELSD

[kan]

Dear All,

I'm in the position to propose a ELSD detector for palm oil & fat analysis. I'm considering Alltech, Varian/Polymer lab and Sedex.

Please give comment(strength and weakness) for the 3 brands mention above in terms of linear range, sensitivity etc...

Please recommend evaporator temperature working range too.

I need more silver ion HPLC with ELSD application paper. The paper I have on hand is from J. Sep. Sci. 2001, 24, 179-185 using Alltech ELSD.

Thanks for helping

[Kostas Petritis]

If your compounds are partially volatile you are better of with a Low Temperature (LT) ELSD from Sedere. You can find the same detector (OEM) from Agilent or Shimatzu.

[kan]

The compounds are non volatile. I even need to add heater for the autosampler since stearin is solidified under ambient temperature.

Anyone have application paper using the silver ion column with ELSD? Please send me a softcopy and I will be very grateful for your helping hand.

Thanks,
Kan

[JGK]

I've used the Alltech 2000ES (Laser source) for phosphatidylcholine and Lysophosphatidylcholine assays and it worked well.

The biggest drawback is the "footprint" which is the equivalent of another HPLC. However this does mean that the dying tube is quite long and this aids the detection.

Do you have a Nitrogen gas generator? If not, you'll need to have space for a few cylinders as the ELSD can use a lot of gas.

You'll also need to have it located in/or under an extract unit or the lab will stink of solvent fumes.

My current lab has the waters model 2424 (lamp source) but we have yet to use it for assays

[kan]

Concerning the nitrogen gas, we are looking at options of utilizing factory nitrogen gas line, cylinder as well as gas generator(Parker).

Do you have any idea of how long a cylinder of pure nitrogen gas can last for the ELSD? Any maintenance for the nitrogen gas generator?

This will help me to justify which option I should go for.

Back to the silver ion HPLC with ELSD, I really appreciate if someone can send me paper on oil and fat.

Thanks

[Bruce Hamilton]

Whenever users need to work with lipid chromatography, they should start with the excellent resources provided by W.W.Christie, whose books, including "Lipid Analysis ", are considered the best starting point for lipid chromatographic techniques.

His website contains a wealth of free information, and his literature summaries will point to suitable documents. There are several free pdf files that practising lipid chemists should have on their computer.

Start with:-
www.lipidlibrary.co.uk/Silver/silver.html

www.lipidlibrary.co.uk/lit_surv/general/hplcsilv.htm

www.lipidlibrary.co.uk/lit_surv/general/elsd.htm

A literature search should also reveal plenty of relevant information.

Bruce Hamilton

[kan]

Thanks Bruce for the link. I did visit the website before and get basic understanding from the writing.

Yet, I do hope to read more application paper in the different aspect study of lipid utilizing silver ion HPLC-ELSD.

Thanks.