Chromatography Forum

Posted: **Tue Sep 16, 2008 7:32 am**

Hi all,

I am running the QQQ (6410 Agilent) in the SRS mode (Scan-Colision-Scan). The spectra has just appeared a ghost peak at 197 amu which is quite high in abundance. The peak now seems to stick in the spectra.
I am not sure where the peak comes from. Any help is appreciated.

Thanks

Posted: **Fri Sep 19, 2008 3:37 pm**

Do you do any chromatography or just infusion and what is your mobile phase or infused solvent? Did you tried to just change the buffer/solvents that you introduce in the MS? What exactly do you mean by "stick" in the spectra?

Posted: **Fri Sep 19, 2008 4:31 pm**

Do you do any chromatography or just infusion and what is your mobile phase or infused solvent? Did you tried to just change the buffer/solvents that you introduce in the MS? What exactly do you mean by "stick" in the spectra?

Thanks for your input. It's just the mobile phase which is a mix of H2O and ACN. I did open the ballast for and it starts to reduce (luckily enough), slowly though.
What I am still confused is if I reduce the mobile flow to about 0.1-0.2ml/min, the peak and all other noises are gone.

Chromatography Forum

Help, ghost peak in QQQ's spectra

Help, ghost peak in QQQ's spectra