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NP sub 2 um columns
Posted: Thu Sep 11, 2008 2:27 pm
by Koen Hollebekkers
Are there suppliers of NPLC columns on bare silica? Particle size can not be lager than 2.5 um.
What is the big difference between a cyano/phenyl NP mechanism and a bare silica column?
Posted: Thu Sep 11, 2008 3:18 pm
by Bryan Evans
Why can the particle size be no bigger than 2.5um?
Re: NP sub 2 um columns
Posted: Thu Sep 11, 2008 3:45 pm
by JGK
Are there suppliers of NPLC columns on bare silica? Particle size can not be lager than 2.5 um.
What is the big difference between a cyano/phenyl NP mechanism and a bare silica column?
Most suppliers will do columns down to 3µm
In normal phase chromatography cyano/phenyl columns are generally less affected by the presence of water in the mobile phase. They will also re-equilbrate faster between injections for gradient methods.
Posted: Thu Sep 11, 2008 6:36 pm
by danko
Hi Koen,
If you need a sub 2μm particle column, why don’t you try the Acquity HILC column?
It might be able to do the job for you.
Best Regards
Posted: Thu Sep 11, 2008 7:30 pm
by Koen Hollebekkers
Why can the particle size be no bigger than 2.5um?
Because we want to speed up the analysis. The “fastâ€
Posted: Mon Sep 15, 2008 12:51 pm
by Fiz
Knauer in Berlin seems to produce NP-columns using 1.8 um material. I remember that I saw somewhere on their website a Tocopherol NP-application in less than 2 minutes.