NP sub 2 um columns

[Koen Hollebekkers]

Are there suppliers of NPLC columns on bare silica? Particle size can not be lager than 2.5 um.
What is the big difference between a cyano/phenyl NP mechanism and a bare silica column?

[Bryan Evans]

Why can the particle size be no bigger than 2.5um?

[JGK]

Are there suppliers of NPLC columns on bare silica? Particle size can not be lager than 2.5 um.
What is the big difference between a cyano/phenyl NP mechanism and a bare silica column?

Most suppliers will do columns down to 3µm

In normal phase chromatography cyano/phenyl columns are generally less affected by the presence of water in the mobile phase. They will also re-equilbrate faster between injections for gradient methods.

[danko]

Hi Koen,

If you need a sub 2μm particle column, why don’t you try the Acquity HILC column?
It might be able to do the job for you.

Best Regards

[Koen Hollebekkers]

Why can the particle size be no bigger than 2.5um?

Because we want to speed up the analysis. The “fastâ€

[Fiz]

Knauer in Berlin seems to produce NP-columns using 1.8 um material. I remember that I saw somewhere on their website a Tocopherol NP-application in less than 2 minutes.