HP 1090 and Agilent 1100 FLD

[guttridm]

I'm currently trying to hook up an Agilent 1100 series FLD to the HP 1090 HPLC machine we have in the lab. The FLD is installed and recognised on the chemstation and all of its parameters can be set during the Edit Entire Method menu. The FLD also shows no Error messages and is ready to run. However, it wont actually run a scan during the HPLC sequence! How can this be done? The only way we are currently able to run a fluorescence scan is when the HPLC is not running a method or sequence. Is it possible to run an FLD scan in sync with the HPLC? If so, how is this done?

Thanks

[Consumer Products Guy]

Did you also connect a remote cable, in addition to the signal cable? Are you sure it's not just software, like the FLD sognal not in "Signal Details"?

[guttridm]

I was maybe thinking it was some sort of software issue on the Chemstation, such as box I have to tick or something along those lines but having searched each menu and subsequent dialog boxes, I can't find anything!

[giacomo56]

You will need to put a stop on the HPLC run, in the sequence table method, so that the peak of interest will be in the FLD cell so that the monochrometer of the FLD can do the scan, then complete the run....pretty hard to do a scan while a peak is moving thru the flow cell.