GPC Mass Recovery

[Jaries]

How do you calculate % Mass recovery on GPC. We are running polyethylene oxide through out GPC columns and i want to make sure everything is coming out and not getting stuck to the columns.

Thank you very much for all the help

[HW Mueller]

Probably the simplest way to do this is to take out the column, inject and detect a standard at the flowrate used to run the column. The areas should be the same as if the column was involved.