Chromatography Forum

Anyone know how to separate alpha and beta farnesene by HPLC (they only differ in a double bond position)?

Thanks in advance.

Anyone know how to separate alpha and beta farnesene by HPLC (they only differ in a double bond position)?

Thanks in advance.

I don't know the structure of this molecule, therefore i give general advances:
1. "RP mode" with Ag+(and other ions) in mobile phase (1-0.1mM), in some cases you can separate this molecules by "simple" RP mode on C18 phase (C% >=16-17), better on C30 using gradient.
2. Quasi-NP mode on silica covered Ag+ and other ions (molecules, for example - riboflavin), and in this case you also can use Hypercarb and Chromalite 5HGN.
3. Simple NP mode (adsorption chromatography) on polar sorbents such as Sil-amino, Sil and other.

Good luck

What is the matrix that you have to deal with?

Sesquiterpenes are usually measured via GC. As this class of components often occurs in mixtures of vast numbers of isomers, I would strongly recommend switching to a GC or GC MS Method.

Any old GC-FID can do the job. (Mine was an Italian Dani 1000)