alpha & beta farnesene separation

[Kwet]

Anyone know how to separate alpha and beta farnesene by HPLC (they only differ in a double bond position)?

Thanks in advance.

[virtu]

Anyone know how to separate alpha and beta farnesene by HPLC (they only differ in a double bond position)?

Thanks in advance.

I don't know the structure of this molecule, therefore i give general advances:
1. "RP mode" with Ag+(and other ions) in mobile phase (1-0.1mM), in some cases you can separate this molecules by "simple" RP mode on C18 phase (C% >=16-17), better on C30 using gradient.
2. Quasi-NP mode on silica covered Ag+ and other ions (molecules, for example - riboflavin), and in this case you also can use Hypercarb and Chromalite 5HGN.
3. Simple NP mode (adsorption chromatography) on polar sorbents such as Sil-amino, Sil and other.

Good luck

[Euro08Soccer]

What is the matrix that you have to deal with?

Sesquiterpenes are usually measured via GC. As this class of components often occurs in mixtures of vast numbers of isomers, I would strongly recommend switching to a GC or GC MS Method.

Any old GC-FID can do the job. (Mine was an Italian Dani 1000)