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Multivitamin Syrup analysis by LC/GC

http://www.chromforum.org/viewtopic.php?t=8498

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Multivitamin Syrup analysis by LC/GC

Posted: Sat May 24, 2008 5:01 am
by Sharif
hi everybody,

how r u? hope fine. i have a problem during analysis of Sevenseas syrup (Multi vitamin syrup). It contain vitamin A, D, E, Pyridoxine, Thiamine, riboflavin and Nicotinamide. Successfully i anlysis them by HPLC. but the problem is there i analysis them by different time. Vitamin A, D and E at one step and vitamin B-Complex in another step. So, i need so much time to anlyze them. is there any process to analysis them at one step by GC or HPLC.

Thanking you

Sharif

Posted: Tue May 27, 2008 4:15 pm
by sassman
Are you saying that you cannot separate them chromatographically? Are you running them all on HPLC? Have you tried adjusting your mobile phase gradient/pH? You could probably get them all in one HPLC run, but it would probably be a long gradient. Might be faster to do seperate isocratic or shallow gradient runs.
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