Multivitamin Syrup analysis by LC/GC

[Sharif]

hi everybody,

how r u? hope fine. i have a problem during analysis of Sevenseas syrup (Multi vitamin syrup). It contain vitamin A, D, E, Pyridoxine, Thiamine, riboflavin and Nicotinamide. Successfully i anlysis them by HPLC. but the problem is there i analysis them by different time. Vitamin A, D and E at one step and vitamin B-Complex in another step. So, i need so much time to anlyze them. is there any process to analysis them at one step by GC or HPLC.

Thanking you

Sharif

[sassman]

Are you saying that you cannot separate them chromatographically? Are you running them all on HPLC? Have you tried adjusting your mobile phase gradient/pH? You could probably get them all in one HPLC run, but it would probably be a long gradient. Might be faster to do seperate isocratic or shallow gradient runs.