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Plot column for nicotine analysis

http://www.chromforum.org/viewtopic.php?t=8345

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Plot column for nicotine analysis

Posted: Sat Apr 26, 2008 4:50 am
by edcvn
Dear all,

We used to analyze nicotine by DB 5 column. Now I just want to change to a PLOT column with more polar stationary. Anyone suggest me on this?
Our analysis is quantitative, not for traced one.

Thank you

Posted: Mon Apr 28, 2008 1:45 am
by chromatographer1
I don't know of any PLOT column which will be useful for your analysis.

If anyone has a solution for nicotine I would encourage them to post it.

Good luck in your analysis. You might wish to review HPLC as a possible replacement technique.

best wishes,

Rod

Posted: Tue Apr 29, 2008 1:44 pm
by AICMM
Edcvn,

Perhaps a cyanophenyl or phenyl siloxane will give you enough pi-pi interaction to better retain nicotine? At the extreme is the BPX-90.

Best regards.
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