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Split Peaks in lc ms ms

Posted: Tue Feb 06, 2018 8:53 am
by Dr.Malik
Hi all,
when I used a new guard for LC column i got split peaks, and when I remove the Guard the peaks appear normal,
Although Guard is new one. and I used more than one Guard.
what might be the reason for that?

Re: Split Peaks in lc ms ms

Posted: Tue Feb 06, 2018 11:43 am
by dblux_
void spaces in guard column

Re: Split Peaks in lc ms ms

Posted: Tue Feb 06, 2018 5:17 pm
by dap
Please check if stationary phase is the same.