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why uracil is used to detect as dead volume of the sytem
Posted: Mon Feb 18, 2008 4:52 pm
by kishore1984
why uracil is used to detect as dead volume of the sytem
Posted: Mon Feb 18, 2008 6:35 pm
by zokitano
Uracil is used for determination of the column void (dead) volume in reverse-phase chromatography, or in other words it is used to determine the dead time (the time for the unretained species to reach the detector).
Uracil is used for this purpose because it is unretained by the RP stationary phases
Posted: Tue Feb 19, 2008 3:43 am
by chromatographer1
If I may add a comment to the answer, it has a good UV response and is easily detected by the commonly used UV detector.
best wishes,
Rod
Posted: Tue Feb 19, 2008 5:06 am
by kishore1984
thank u
Posted: Tue Feb 19, 2008 11:20 am
by HW Mueller
There have been extensive discussions on determination of to (tm). One needs to be careful, uracil is retained by some columns under some conditions.