Chromatography Forum

Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/

Valproic acid: peak tailing problem

http://www.chromforum.org/viewtopic.php?t=7573

Page 1 of 1

Valproic acid: peak tailing problem

Posted: Wed Jan 02, 2008 7:31 am
by jbshan
Dear friends,
Am doing the method development of Valproic acid. IK have problems with the base line (very high ) and the peak shape is bad with severe tailing. I have used almost all cominations olf LCMS buffers as Mp and tried with different columns. The method is a SIM.
If anyone has any idea about the method, please help me

shan

hi

Posted: Wed Jan 02, 2008 5:19 pm
by alpesh
i would ask u first the basic question about valproic acid. it is not fragmenting in LCMSMS. so can u please tell me which ion you have selected?? As we have done valproic acid as PsedoSRM method in LCMSMS.

Posted: Thu Jan 03, 2008 12:12 am
by SIELC_Tech
One of our customers had same problem with tailing of valproic acid on a regular C18 column. We developed the following method for LC/MS:
http://www.sielc.com/compound_207.html

regards,

Vlad

Posted: Thu Jan 03, 2008 3:29 am
by jbshan
dear aplesh

Even am using a pseudo SRM for the amu 142.9. Could you please get me some literature which is helpful.

Posted: Thu Jan 17, 2008 2:46 am
by Stryder08
First: What ESI mode are you using? As VA is an acid, you should use Negative Mode...
All times are UTC
Page 1 of 1
Powered by phpBB® Forum Software © phpBB Limited
https://www.phpbb.com/