Valproic acid: peak tailing problem

[jbshan]

Dear friends,
Am doing the method development of Valproic acid. IK have problems with the base line (very high ) and the peak shape is bad with severe tailing. I have used almost all cominations olf LCMS buffers as Mp and tried with different columns. The method is a SIM.
If anyone has any idea about the method, please help me

shan

[alpesh]

i would ask u first the basic question about valproic acid. it is not fragmenting in LCMSMS. so can u please tell me which ion you have selected?? As we have done valproic acid as PsedoSRM method in LCMSMS.

[SIELC_Tech]

One of our customers had same problem with tailing of valproic acid on a regular C18 column. We developed the following method for LC/MS:
http://www.sielc.com/compound_207.html

regards,

Vlad

[jbshan]

dear aplesh

Even am using a pseudo SRM for the amu 142.9. Could you please get me some literature which is helpful.

[Stryder08]

First: What ESI mode are you using? As VA is an acid, you should use Negative Mode...