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splitter advice
Posted: Fri Dec 14, 2007 5:41 am
by mariojon
Hello,
Well here is the problem I am using LCMS with ESI probe . The Mass system is waters ZQ4000 and what I want to ask is what type of splitter is best . eg low pressure , high pressure splitter valves. where i need only 0.2ml to flow to the mass . Will PEEK material or steel be better.
Also please suggest me good post column switching valve( Company).
Thank you
John
Posted: Fri Dec 14, 2007 6:55 am
by bert
Hi John,
Why dont you use columns with smaller ID's, so you can work with lower flows?
Re: splitter advice
Posted: Sun Dec 16, 2007 6:04 am
by vicou_chen
Hello,
Well here is the problem I am using LCMS with ESI probe . The Mass system is waters ZQ4000 and what I want to ask is what type of splitter is best . eg low pressure , high pressure splitter valves. where i need only 0.2ml to flow to the mass . Will PEEK material or steel be better.
Also please suggest me good post column switching valve( Company).
Thank you
John
in my opinion,you should use a PEEK material splitter.because PEEK material can preferably resist your solvent and sample properties.
Posted: Mon Dec 17, 2007 7:53 am
by H.Thomas
I have experimented with different setups for flow splitters for our Shimadzu LCMS 2010. I was nor very successful with homebrew solutions (i.e. a tee with different lengths of PEEK capillaries). The reason is, that the backpressure of the ESI changes, and so the split ratio was not constant over more than a few runs.
Finally I bought an ASI Quick-Split variable flow splitter (
http://www.hplc-asi.com/pdf/FSPB2003np.pdf) that was very expensive but worked satisfactorily so far.
Regards, Hartmut