Problem with autotune in MSD 5973

[astroguille]

Hi.
First, I'm sorry for my bad english. I hope that you understand me.

I work a MSD 5973.
Two weeks ago, I noticed that the chromatograms was flat.
Then, I executed the autotune, but this did not finish.
I cleaned the ion source and the HED, but nothing changed.
After that, I removed the column and placed a new column.
In this moment, I can execute the autotune, but the results are not good:

EMVolts: 2906
#peaks: 437
Mass 69 precursor: 10.42
Mass 219 precursor: 52.08
Mass 502 precursor: 51.23
Position of isotope mass 70: low
Position of isotope mass 220: low
Ratios of isotope masses: horrible

Can somebody help me?

[AICMM]

Astroguille,

Wid219 is the width of the 219 ion. Since it is in the middle of the PFTBA tune and a strong responder, adjusting the width of that ion really can affect the outcome of the tune.

Cleaning the source was a good idea. Cleaning the HID was probably not. I would start by replacing your HED and re-running an autotune. Then post the autotune and there are a number of people who can help you resolve what ever issues remain. With the number of peaks detected, I think you are only looking at noise because the HED may be fried.

By the way, it could have been as simple as changing from filament 1 to filament 2.

Best regards.

[Ron]

The EM voltage is very high. How old is the electron multiplier? It could be at the end of its useful life.

[Rick]

Your EM voltage is very high....habitually, you need to change your EM at this moment but beforee change your EM, verify:
1-If your calibration vial is not empty
2-If you select the good tune mode.
3-If you have not a leak with ion gauge.
4-Disconnect your column and cap your ms interface, it is more easy to diagnostic a problem with this manner...tune again with no column
5-Change your EM