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C18 column swich from 65% ACN to 65% MeOH

http://www.chromforum.org/viewtopic.php?t=7311

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C18 column swich from 65% ACN to 65% MeOH

Posted: Wed Nov 14, 2007 6:31 pm
by genggeng
C18 column swiched directly from 65% ACN 35% H20 to 65% MeOH 35% H20. Is it Ok for the column?

Thanks!

Posted: Wed Nov 14, 2007 7:01 pm
by Consumer Products Guy
should be fine; your pressures will be less with ACN, and most likely your analytes will elute a little sooner with ACN if the percent organic remains the same

Posted: Wed Nov 14, 2007 9:14 pm
by genggeng
Thanks!!
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