2 ml/min for a 3um particle size column?

[zimanli]

Greetings.

We are validating a method transferred from another company, which has been approved by FDA
they use ODS inertsil 10cm*4.6mm*3um column. the column flow is 2ml/min. is it too fast?
how is column flow related with the particle size d ID?

Thank you in advance!

[Consumer Products Guy]

It's OK if your pressure stays OK and doesn't overpressure.

[duantech]

It shall be OK. The column is kind of short.

[zokitano]

how is column flow related with the particle size d ID?

Flow rate is related with the column internal diameter:

Flow rate = f (diameter^2) ; f - function

e.g. if the column I.D is 4.6mm at 2mL/min flow, then the flow that should be used if you change the column with another with 2.1mm I.D is:

(4.6mm/2.1mm)^2 = 4.8 (rounded to 5)
The flow rate will be 2mL/min:5 = 0.4mL/min for the narrower column.

Regards

[Uwe Neue]

I have run many methods on a 4.6 x 10 x 3.5 micron column at this flow rate. For water, I expect a pressure around 3000 psi, less for mixtures of acetonitrile and water. Only for a methanol-water mixture, you are pushing the limits.