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Peak fronting - chiral method

http://www.chromforum.org/viewtopic.php?t=7133

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Peak fronting - chiral method

Posted: Thu Oct 18, 2007 12:40 pm
by mojo
We are transferring a chiral method for determiniing the S isomer content of an R isomer API. The column is a Chirex penicillamine and the mobile phase is 65% CuIIpentahydrate sln with 35% ACN. Std is prep'sd in the mobile phase. We are witnessing significant peak fronting. Changing inj volume does not help.

Posted: Thu Oct 18, 2007 1:37 pm
by danko
Maybe you’re dealing with column void volume/headspace.
Try to reverse the column and see if the fronting disappears.

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