Chromatography Forum

Posted: **Thu Sep 27, 2007 2:17 pm**

hallo,
Can somebody tell me why I used MeOH to extract Aflatoxin obtained often more than 100% recovery (about 150%) than use ACN. The methode and HPLC system are the same. The peaks are also good, no signs of bad seperations. thanks.

Zhen

Posted: **Thu Sep 27, 2007 5:32 pm**

What does your matrix blank look like? If you spike at two or three levels, and graph the area vs spike level, does it have a non-zero intercept?

Posted: **Fri Sep 28, 2007 9:48 am**

my blank sample has no detection of alfatoxin. But it's a good idea to try with different spike level which I didn't tried so far. thanks.