recovery

[yzhen77]

hallo,
Can somebody tell me why I used MeOH to extract Aflatoxin obtained often more than 100% recovery (about 150%) than use ACN. The methode and HPLC system are the same. The peaks are also good, no signs of bad seperations. thanks.

Zhen

[Mark Tracy]

What does your matrix blank look like? If you spike at two or three levels, and graph the area vs spike level, does it have a non-zero intercept?

[yzhen77]

my blank sample has no detection of alfatoxin. But it's a good idea to try with different spike level which I didn't tried so far. thanks.