EDA.HCl Analysis on Ion-Pair Chromatography

[Chandresh K. Soni]

Hi to all
My Problem is the analysis of EthylenDiamine Hydrochloride salt on HPLC.
We Use 0.02M Heptane Sulphonic acid Salt as ionpair reagent and found Little response at 200nm But When We Inject HCl the Same R.T. is found . My Querry is That this peak is Realy of EDA.HCl or only due to HCL.
Thanks in Advance for your Valuable Suggestion.

[HW Mueller]

There should be very little, if any, EDAHCl or HCl in your aqueous solution. You probably see Cl-.

[Chandresh K. Soni]

Thanks for Your Valuable Suggestion.
I Think this is Due to n to n* transitions of Free Electron of Chlorine atom. But is there any possibility of Derrivitizaton of EDA. HCl .
Again Thanks

Dr. Chandresh K.Soni

[SIELC_Tech]

Ethylenediamine has no UV activity. Whatever you see is base line disturbance caused by injection. You need to use ELSD or derivatize. There are bunch of reagents to derivatize amino groups.
Alternatively you can use ELSD with no ion-pairing reagent in the mobile phase (although I see some people arguing that TFA is IP):
http://www.sielc.com/compound_106.html
http://www.sielc.com/compound_140.html (please note that it is 10 mm column in first application)

[HW Mueller]

Hm, havn´t seen the n transition terminology used in elementary ions, but the detector doesn´t care anyway.
SIELC_Tec, do you have a UV spectrum of EDA?

[Mark Tracy]

If you don't mind getting an ion chromatograph, the Dionex IonPac CS18 column separates ethylenediamine and related substances quite nicely using conductivity detection.

On the other hand, if all you want is assay, perhaps titration or elemental analysis would be enough.