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Detector SN4000 problems

http://www.chromforum.org/viewtopic.php?t=6640

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Detector SN4000 problems

Posted: Wed Aug 15, 2007 2:08 pm
by Rganga
Hi helpers
I have a problem with the detector. I am reading at 3 wavelength, 234, 280 and 300. and my chromatograms are not starting from 0 mAU, they start from 18, 210 and 200 mAU. I did the `review run for about 30 min. What could be the problem and how I could resolve it.

Thanks very much

Posted: Wed Aug 15, 2007 4:14 pm
by Mark Tracy
I assume you are autozeroing the detector sometime before the start of run. Check that the detector signal is actually stable at the time that the autozero is executed.

Posted: Wed Aug 15, 2007 6:34 pm
by DR
I assume you are autozeroing the detector sometime before the start of run. Check that the detector signal is actually stable at the time that the autozero is executed.
...stable at the lowest wavelength...
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