Page 1 of 1
BAD PEAK SHAPE
Posted: Fri Aug 03, 2007 7:30 am
by AMIT KUMAR JAIN
Dear ALL
We are analysing the acetic acid as residual solvent in one of the API.
We are not getting good peak shape at std. concentration,while injecting high concentration peak shape is ok.
can any one suggest solution.
regards
AKJ
Posted: Fri Aug 03, 2007 7:56 am
by zokitano
Dear Amit
Please give us more information about the chromatographic system, carrier, flow, column, type of API etc.
Regards
Posted: Fri Aug 03, 2007 2:03 pm
by chromatographer1
To measure acetic acid by GC and from a pharmaceutical product matrix is not trivial.
The fact that you are getting a peak at all at low levels is commendable.
Any active site on your column, detector, or in your injection port will cause absorption of the acetic acid. Replacement of your injection liner often and use of the proper phase for your column will improve your analysis.
Acetic acid is usually done by ion chromatography or by HPLC.
There are many published methods and companies that sell HPLC columns that will be happy to assist you.
best wishes,
Rod