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ESI or APCI
Posted: Mon Aug 30, 2004 8:04 pm
by jan
What ionization method will be tried out first when one wants to do LC/MS analysis? Will it based on molecule polarity? Thanks.
Posted: Tue Aug 31, 2004 4:05 pm
by Basil
Hi Jan,
I
hardly strongly recomend you to see what's on structure: If there is a N in the structure and it's not to big (high molecular weight) I would begin with ESI in positive mode.
But it also depend in the HPLC mobil phase that you are using because some compounds could produce a bad spray with ESI........
More information will be usefull to help you.
Bye....
Posted: Tue Aug 31, 2004 6:46 pm
by Kostas Petritis
Hi Jan,
I started by writing a long reply of all the factors that you need to consider but that is not what you asked. So...
Personally I start almost always with ESI except if I see that the molecule seems quite hydrophobic and without any chargable functional groups. If you have a molecule which response is the same with both ionisation techniques then I would go with APCI as it has been proven that you have less ion suppression from matrix interferences...
Kostas
Posted: Wed Sep 01, 2004 7:59 am
by Basil
Ops a big mistake !!
I hardly recomend you to see what's on structure
It should said: I
strongly recomend you to study the structure to decide the kind of ionization that you are going to use.....
I agree with Kostas reply....
Posted: Wed Sep 01, 2004 3:17 pm
by tom jupille
To Basil: the new Forum software allows you to edit or delete your own posts (to correct for typos!). Look for buttons in the upper right of the message box.