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- Posts: 6
- Joined: Tue Apr 24, 2007 12:44 pm
Dear Friends,
I am analysing Acarbose. Column is Thermo Hypersil APS-2 column. Mobile Phase is Acetonitrile:Buffer (75:25). For a new column RT is about 16 mins. But while running a sequence, RT is shifted (decreased slowly). At the starting of the sequence RT is 16.6 mins. After that slowly RT is decreased and at the end of the sequence , i got 15.3 mins. why this difference? and where is the problem? Can anyone help me.
Buffer Prep: 0.6 g KH2PO4 and 0.35 g anhy. Na2HPO4 in 1000 ml of water.
And how to maintaining this APS-2 column? For washing this column is water only suitable. ? Please help me.
I am analysing Acarbose. Column is Thermo Hypersil APS-2 column. Mobile Phase is Acetonitrile:Buffer (75:25). For a new column RT is about 16 mins. But while running a sequence, RT is shifted (decreased slowly). At the starting of the sequence RT is 16.6 mins. After that slowly RT is decreased and at the end of the sequence , i got 15.3 mins. why this difference? and where is the problem? Can anyone help me.
Buffer Prep: 0.6 g KH2PO4 and 0.35 g anhy. Na2HPO4 in 1000 ml of water.
And how to maintaining this APS-2 column? For washing this column is water only suitable. ? Please help me.
shanmuga
