conditions for compound insoluble/unstable in water?

[rohitdrl]

dear all
I am looking to develop an hplc method for an ester prodrug of an antibiotic. it has a pKa of 8.4 and is basic in nature. It is not soluble in water (1%) and has stability problems in aqueous solution. Kindly suggest me solvents to dissolve the drug as well as mobile phase for performing HPLC (with photo diode array detector)

reply asap

[tom jupille]

We're not mind-readers. Before we can suggest a solvent and conditions, it would help if you could tell us what it is soluble in!