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Unfamiliar with ELSD

http://www.chromforum.org/viewtopic.php?t=5946

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Unfamiliar with ELSD

Posted: Wed Apr 25, 2007 2:26 pm
by CSV
Hi, everyone!
I'm trying to assay oleic acid and monoolein in a farmaceutical product using RP chromatography with ELS detection. I'm not very familiar with this detector and when I found out that the precision was poor I supposed it could be due to detector fluctuations. I added palmitic acid (eluting between the analytes of interest) but its area remains constant while the others vary some between injections.
I would very much like some suggestions of how to increase the precision and what to think of when doing method development on an ELSD.

Thanks

Posted: Wed Apr 25, 2007 3:32 pm
by SIELC_Tech
Check your ELSD temperature. These carboxylic acids can parially evaporate in ELSD thus reducing amount of acid you see. Try to drop temperature to 35*C in ELSD.
We had this problem when we developed a method for C12, C14 and C16 carboxylic acids.

regards,

Vlad
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