Unfamiliar with ELSD

[CSV]

Hi, everyone!
I'm trying to assay oleic acid and monoolein in a farmaceutical product using RP chromatography with ELS detection. I'm not very familiar with this detector and when I found out that the precision was poor I supposed it could be due to detector fluctuations. I added palmitic acid (eluting between the analytes of interest) but its area remains constant while the others vary some between injections.
I would very much like some suggestions of how to increase the precision and what to think of when doing method development on an ELSD.

Thanks

[SIELC_Tech]

Check your ELSD temperature. These carboxylic acids can parially evaporate in ELSD thus reducing amount of acid you see. Try to drop temperature to 35*C in ELSD.
We had this problem when we developed a method for C12, C14 and C16 carboxylic acids.

regards,

Vlad