acetate and lactate by IC

[bpjackso]

I've been trying to separate aceteate and lactate by IEC using a Dionex AS11 column and a dilute (< 1 mM) NaOH eluant with conductivity detection. The peaks are poorly resolved and overlapping at higher concentrations. The column literature suggests that this separation should be possible.

Does anyone have any suggestions?

[Mark Tracy]

Is yours an AS11 or an AS11-HC? The AS11-HC is much better for this application. Have you looked at the product datasheet?
http://www1.dionex.com/en-us/webdocs/41 ... HC_V15.pdf
It compares the two columns in Figure 1. Also, try raising the temperature to 30 °C.

You can also consider the just-introduced Acclaim Mixed-Mode WAX. The user's guide is at:
http://www1.dionex.com/en-us/webdocs/48 ... olumns.pdf