processing a sample for HPLC

[tracy]

I have an hydrolysate in 6N HCl that I have to neutralize and precipitate and I don't know with what and what concentration with. Could anyone help me to find this out? I have to run it on HPLC so it has to be clean before I derivitize it for amino acids. I would like to find out how the chemistry works too could you lead me to any sights to look at to see how it works for further processing methods I may need to do? Thanks for yur Help Tracy

[Mark Tracy]

Since HCl is volatile, the more usual approach is to dry the sample either by freeze-drying or rotary evaporator. Then reconstitute in a compatible buffer. Then filter.

If you want to neutralize, use 6M NaOH. That is messy because it leaves a lot of NaCl that can interfere with later processing. Also, you don't want to titrate each sample if you can avoid it.