IP reagents + buffers

[Philip]

Can anyone tell me what the effect of IP reagents (e.g. alkylsulfonates) and buffers (e.g. Ammonium Acetate/Glacial Acetic Acid) + organic, mixed, might be...? Suppose it might be a better question to ask if there is any adverse or potential positive system mixing IP reagents + buffer...?

Thanks alot!

Regards,

Phil

[danko]

Hi Philip,

No problem, as long as the plan is thought through. By that I mean: You should ensure that your analyte is positively charged while the alkylsulfonate is negatively charged. So start with finding the pKa for these and adjust the buffer accordingly. Remember, the buffer pH should be adjusted in accordance with its buffer capacity range, i.e. pKa ± 1. Acetate’s pKa is 4.75.

Good luck

[4ugirish]

Can anyone tell me what the effect of IP reagents (e.g. alkylsulfonates) and buffers (e.g. Ammonium Acetate/Glacial Acetic Acid) + organic, mixed, might be...? Suppose it might be a better question to ask if there is any adverse or potential positive system mixing IP reagents + buffer...?

Thanks alot!

Regards,

Phil

Dear philip I am not clear about your question, kindly elaborate it

[tom jupille]

Make sure that everything is soluble when mixed (good idea to try mixing typical proportions in a test tube to check).

[Dan]

Phil,

As an example of what Tom wrote: don't use the potassium salt of a buffer reagent if the IP reagent is SDS. You will get a precipitate.

Regards,
Dan

[ym3142]

As an example of what Tom wrote: don't use the potassium salt of a buffer reagent if the IP reagent is SDS. You will get a precipitate.

Dan, I donot understand your claim since I have used KH2PO4 and SDS all the time. Thanks for explaination