Page 1 of 1
Importance of Y-Intercept in Method Validation
Posted: Tue Nov 07, 2006 7:51 am
by grreddy
Dear Friends
What information we will get from Y-Intercept in method Validation
G.R.Reddy,
AR&D
BIOCON Limited,
India
Posted: Tue Nov 07, 2006 7:56 am
by Mattias
Quick answer:
Intercept above zero: interferences, blank peaks
Intercept below zero: adsorption of substance
Posted: Tue Nov 07, 2006 8:28 am
by grreddy
Thank you very much
Is there any articles or publications on this
Posted: Tue Nov 07, 2006 9:06 am
by Mattias
There can be of course be other reasons for intercept not equal to zero (e.g not linear response). But if the respones is linear the explainations above are quite likely.
Regarding a reference, I think you can find it in a good LC book like Snyder-Kirklands "Introduction to modern liquid chromatography".
Posted: Tue Nov 07, 2006 6:37 pm
by Mark Tracy
Also, you should compute the confidence interval around the intercept. If it includes zero, then you can consider the intercept to be zero for practical purposes.