LC/MS

[kvalliappan]

Hi!

I would like to know whether the method development apporoach/protocol that we follow for HPLC can be appled to develop a method for LC/MS as well or do we have any special precuations to be taken?

Thanks for your time.

[tom jupille]

Depends on what that protocol is!

The general approach will be the same (get retention first, then focus on selectivity, finally tweak efficiency as necessary). Specific differences will include:

• - the resolution requirements for LC-MS are generally less stringent than for LC-UV
  - mobile phase buffers/additives, etc. must be volatile and compatible with the ionization technique used
  - mobile phase flow is typically restricted by the need to vaporize the solvent (hence smaller columns and lower flow rates)
  - smaller columns mean smaller injection volumes and the need to be more careful about extra-column volume generally.

[kvalliappan]

Dear Mr.Tom Jupille.

Thanks for the information.

I would like to the site where I could get the best information on LC/MS theory and the practical aspects (tutorials/reviews/presentations).

[tom jupille]

Click on the FAQ link near the upper left of the screen (under the Chromatography Forum logo) and drill down to the "additional information" page.

[kvalliappan]

Click on the FAQ link near the upper left of the screen (under the Chromatography Forum logo) and drill down to the "additional information" page.

Thanks