betaine

[vicentexxx]

Hi everyone,

could someone advice me a suitable method for analysis of betaine (HPLC or other methods)?
i'm trying an UV method with reinecke salt but validation is quiet plodding and difficult...
thank you in advance
vincenzo

[SIELC_Tech]

Here is method for betaine. You have better chances with ELSD/LC/MS detection. For low UV you can use sulfuric or phosphoric acid (but betaine has limited UV activity)

http://www.sielc.com/compound_197.html

This analysis is performed on 4.6x50 mm column. If you have other compounds in the mixture you might consider a longer column (100 or 150 mm)

[Bryan Evans]

Hey Vincenzo -

Here is a chromatogram of betaine using our Unison UK-Silica column:

http://www.imtakt.com/TecInfo/TI191E.pdf

[Uwe Neue]

I would use HILIC on a silica column. Roughly 70 to 80% acetonitrile, 30 to 20 % water.

[Einar Ponten]

See Polymer Laboratories Application Note:

TB1074: Quantification of Betaine in Chinese Lycium using Hydrophilic Interaction Chromatography (HILIC) and ELS Detection.

http://www.polymerlabs.com/cgi-bin/fill ... chbull.htm



Do I need to say that they use ZIC®-HILIC...

[Bart]

There is a very simple method available using ligand exchange columns:

http://chromatography.transgenomic.com/ ... .asp?tab=2

Bart Poulsen

Transgenomic, Inc.
Director, Chromatography Product Management

[Bart]

One of my colleagues told me my link did not work. It will take a bit longer to get there, but just in case, you can start at our home web site and find it after some searching: chromatography/carbohydrate analysis/Coregel 87K/chromatogram.

www.transgenomic.com

Bart

[vicentexxx]

a question:

is possible in your opinion to use a conventional c18 column at low pH values (2.1-2. and a ion pair reagent like eptanSO4?at theese pH betaine should be a cation so if i prepare mobile phase with ion pair reagent it could be ok...
what about??
thank you

[Einar Ponten]

There may be a possibility that you can suppress the carboxylic acid group and retain it as a ion pair with heptan sulfonate on a RP column.

However, I suspect that you will have difficulties with the detection since UV absorbtion by betaine is rather low and the ion-pairing is not compatible with ELSD or MS detection.