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Determining peak purity when running a gradient method

Posted: Thu Oct 05, 2006 3:30 pm
by Karen Lee
Can peak purity be accurately determined if the API's are analysed using a gradient method? If so how do you select a representative portion of baseline noise for the peak purity calculation required when analysing using Empower software?

Posted: Thu Oct 05, 2006 3:53 pm
by PJ8
There is full instructions in the Empower training guide supplement for PDA detectors from page 32.

Not sure whether I am allowed to paste that here, but if you have empower the training guides should be available to you somewhere.

Cheers, Pat.

Posted: Thu Oct 05, 2006 4:05 pm
by PJ8
User guide is also on Waters website, you have to set up an account on the website, which is free.

http://www.waters.com/WatersDivision/ce ... N%2D5KULPD

you want UG0000191 third from bottom and page 47 has a walk through guide (better than the old one I found on our company database.)

Regards, Pat.