Determining peak purity when running a gradient method

[Karen Lee]

Can peak purity be accurately determined if the API's are analysed using a gradient method? If so how do you select a representative portion of baseline noise for the peak purity calculation required when analysing using Empower software?

[PJ8]

There is full instructions in the Empower training guide supplement for PDA detectors from page 32.

Not sure whether I am allowed to paste that here, but if you have empower the training guides should be available to you somewhere.

Cheers, Pat.

[PJ8]

User guide is also on Waters website, you have to set up an account on the website, which is free.

http://www.waters.com/WatersDivision/ce ... N%2D5KULPD

you want UG0000191 third from bottom and page 47 has a walk through guide (better than the old one I found on our company database.)

Regards, Pat.