1 point calibration for chemstation

[ramman78]

I work with a multiple sclerosis group, we are trying to figure out which cannabinoids work best and potency. To help this I purchased an agilent 1100 hplc, I have found walk throughs for everything but how to calibrate. I am using chemstation with a restek standard 1000 ul/ml THCA. Specifically the sample info page (dilition, sample amount etc). Can anyone help? Thanks in advance.

[BostonFSE]

you can just search the web for this info, I would do a 5 point curve and aim for >0.999 R2. Just dilute the standard you have
http://www.restek.com/chromatogram/view/LC_GN0553

[Consumer Products Guy]

To help this I purchased an agilent 1100 hplc, I have found walk throughs for everything but how to calibrate. I am using chemstation with a restek standard 1000 ul/ml THCA. Specifically the sample info page (dilution, sample amount etc). Can anyone help? Thanks in advance.

After validations, we did 1-point calibrations with Agilent HPLCs, mostly Revision A software. In the calibration table, we entered the active concentration in grams/ml, and set as %Amt or something similar in a different part of Data Analysis, and saved Method.

Then in Sample Table, the amount of sample was entered in grams. In Dilution, the number of ml of solvent was entered. If there was a subsequent dilution, say 5.0ml diluted to 100ml, then that factor ("20") would go into the multiplier.

To change results to ppm, we'd through an additional 10,000 into the Multiplier column.

So we'd get a single results page, with results in percent, or in ppm; note that when we did as ppm, the Heading of the column would still say "%".

I think I remembered most of it, I'm retired now! I think there's an Agilent PDF on doing this though; the first time I did such on a computer - early 1990s - I had to call HP/Agilent at least 3 times to get it set up, their instruction manual didn't help much.