suitable method for Carbidopa and Levodopa

[venkatpadala]

Dear all,

Here we are developing a method for Carbidopa and Levodopa in human plasma using LCMSMS. But still we are not getting good recoveries for above. We tried in many methods.

please advise us for improving recovery

regards
venkateswarlu

[james little]

Did you consider the recovery could just be matrix suppression of the analyte in the plasma versus standard response in the standard?

Good book to get is

1. H. Mei in: W. Korfmacher (Ed.), Using Mass Spectrometry for Drug Metabolism
Studies, CRC Press, Boca Raton, FL, 2005, p. 103 (Chapter 4).
see

http://www.amazon.com/gp/product/084931 ... e&n=283155


I have some info at my web site on matrix effects..

http://users.chartertn.net/slittle/