Column for highly aqueous mobile phase

[Prometheus]

Dear All

Can you recommend analytical column suitable to a highly aqueous mobile phase,less than 5% organic in HPLC analysis?

[frost47]

We've used a YMS-ODS-AQ 2.0 x 150 for two compounds that were highly hydrophilic. Organic was run at 4% with no problems.

[Bryan Evans]

You can try out Unison UK C18 - it's suitable for 100% aqueous
mobile phase, pH durable, and gets great column efficiency.

[Mark Tracy]

The Dionex Acclaim PolarAdvantage and PA2 are designed for 0-100% aqueous conditions. The Acclaim 300 C18 will work over the range 0-95% aqueous; this is a wide-pore type column. See http://www1.dionex.com/en-us/columns_ac ... p5669.html

[JZeszotarski]

One of our methods uses Supelco's Ascentis RP-Amide and we run a gradient starting at 1% organic.

Phenomenex has similar column technologies as well (Synergi Polar-RP, Synergi Fusion-RP).

Earlier in my career I had good luck with ODS-Aq as mentioned by an earlier post.

Bottom line - there should be a number of options available to you... Best of luck.

Jon

[ChemicalBond007]

I believe the Discovery RP C16 Amide is one generation behind the above mentioned Ascentis Amide. This may mean a lower price compared to the newer Ascentis.

According to Supelco technical support you can run 100% aqueous (on the Discovery). The lowest organic we have used is 5% (MeOH) with very good results.

[Kostas Petritis]

Another option would be to use C30 columns that have been shown not to have the dewetting problems the C18 columns have... Of course it always depends on your analytes...

[Prometheus]

Hi All,

Thank you for all your suggestions.

[Norikazu Nagae]

Dear All

Can you recommend analytical column suitable to a highly aqueous mobile phase,less than 5% organic in HPLC analysis?

You can obtain the method that any reversed-phase column shows high reproducible retention under 100% aqueous conditions if you see the following website:

http://develosil.net/HPLC2005WATER.pdf
http://www.americanbiotechnologylaborat ... 004ena.pdf
http://www.lcgcmag.com/lcgc/data/articl ... rticle.pdf

[Rafael Chust]

There are several columns in the market for 100% aqueous mobile phase.

I have experience with Phenomenex Synergi Hydro-RP - that replaced Aquasil C18.

[ghie malig]

Hi All,

Thanks for your recommendation especially to the website refer by Norikazu Nagae. I learn a lot from your article.