Chromatography Forum

hello....this my first time in this forum...u r the only way to solve my problem!
Here it is:
I'm an analytical researcher and in this period I’ve been dealing with HPLC analysis of natural products, in particular rutin.

My problem regards the analysis of rutin, in fact I tried to inject several rutin samples (also rutin sigma purum 95% with HPLC method) with several method and column but I can’t obtain an acceptable peak form.
I read a lot of publications about HPLC determination of flavonols in which rutin is mentioned but i can't fit the this performances.

It seems that rutin is a pool of similar compounds that eluate with similar retention time but this idea is not reinforced by my trials.
The only column that gave me a good performance is a an Astec amino, appropriate for carbohydrates analysis but not interesting for the other active ingredients of the tablet so I can’t use it.

So, what about this problem? I think your opinion could be very important.

I thank you very much anyway



Vincenzo Mele

I've had some promising results for natural products using a carbon (graphite) column - Hypercarb from Thermo. It provided separation of some epimers that conventional reverse phase or normal phase separations could not. Just be sure to read their product notes thoroughly because very strong mobile phases are often needed.

Hope that helps,
Jon

Ciao,
we are doing analysis of rutin and quercetin (similar like rutin), try this!
mobile phase: 18% acetonitrile
82% 0,01M Na2HPO4, pH 2,6
isokratic,
column: C18, 250x4,5x5 (for us: thermohypersil, but i think, it will be good with others C18 too)
rutin RT=cca 8,5min
UV detection, 260nm
and it works!

Ciao
Majlan