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Column for highly aqueous mobile phase
Posted: Thu Jul 13, 2006 9:09 am
by Prometheus
Dear All
Can you recommend analytical column suitable to a highly aqueous mobile phase,less than 5% organic in HPLC analysis?
Posted: Thu Jul 13, 2006 4:08 pm
by frost47
We've used a YMS-ODS-AQ 2.0 x 150 for two compounds that were highly hydrophilic. Organic was run at 4% with no problems.
Posted: Thu Jul 13, 2006 4:40 pm
by Bryan Evans
You can try out Unison UK C18 - it's suitable for 100% aqueous
mobile phase, pH durable, and gets great column efficiency.
Posted: Thu Jul 13, 2006 5:20 pm
by Mark Tracy
The Dionex Acclaim PolarAdvantage and PA2 are designed for 0-100% aqueous conditions. The Acclaim 300 C18 will work over the range 0-95% aqueous; this is a wide-pore type column. See
http://www1.dionex.com/en-us/columns_ac ... p5669.html
Posted: Fri Jul 14, 2006 3:47 pm
by JZeszotarski
One of our methods uses Supelco's Ascentis RP-Amide and we run a gradient starting at 1% organic.
Phenomenex has similar column technologies as well (Synergi Polar-RP, Synergi Fusion-RP).
Earlier in my career I had good luck with ODS-Aq as mentioned by an earlier post.
Bottom line - there should be a number of options available to you... Best of luck.
Jon
Posted: Fri Jul 14, 2006 9:21 pm
by ChemicalBond007
I believe the Discovery RP C16 Amide is one generation behind the above mentioned Ascentis Amide. This may mean a lower price compared to the newer Ascentis.
According to Supelco technical support you can run 100% aqueous (on the Discovery). The lowest organic we have used is 5% (MeOH) with very good results.
Posted: Sat Jul 15, 2006 12:05 am
by Kostas Petritis
Another option would be to use C30 columns that have been shown not to have the dewetting problems the C18 columns have... Of course it always depends on your analytes...
Posted: Mon Jul 17, 2006 8:38 am
by Prometheus
Hi All,
Thank you for all your suggestions.
Re: Column for highly aqueous mobile phase
Posted: Tue Jul 25, 2006 11:05 am
by Norikazu Nagae
Dear All
Can you recommend analytical column suitable to a highly aqueous mobile phase,less than 5% organic in HPLC analysis?
You can obtain the method that any reversed-phase column shows high reproducible retention under 100% aqueous conditions if you see the following website:
http://develosil.net/HPLC2005WATER.pdf
http://www.americanbiotechnologylaborat ... 004ena.pdf
http://www.lcgcmag.com/lcgc/data/articl ... rticle.pdf
Posted: Fri Jul 28, 2006 7:10 am
by Rafael Chust
There are several columns in the market for 100% aqueous mobile phase.
I have experience with Phenomenex Synergi Hydro-RP - that replaced Aquasil C18.
Posted: Fri Jul 28, 2006 11:11 am
by ghie malig
Hi All,
Thanks for your recommendation especially to the website refer by Norikazu Nagae. I learn a lot from your article.