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iron(III) EDTA complex
Posted: Tue Jul 11, 2006 4:54 pm
by Louise77
Has anyone analysed iron(III) EDTA complex on HPLC? Any recommendation on the column? Zorbax?
Posted: Tue Jul 11, 2006 6:43 pm
by Albany-12303
We test for EDTA by complexing it with copper (we put a small amount of CuSO4 in the dilutent and mobile phase). You can probably analyse Iron by putting some EDTA in the diluent/mobile phase
Something like this:
Column: Hamilton PRP-X100 ,10µ (250mm x 4.1mm)
Detector: 254nm UV
Inj Vol: 50µL
Flow: 1.0mL/min
Mobile Phase: 1M Sulfuric Acid 2.0mL
Phosphoric Acid, conc 2.0mL
Purified Water, USP 900mL
Methanol 100mL
0.1M Cupric Sulfate Solution 5.0mL
(Mix well and filter through a 0.45 micron membrane filter)
Retention Time of Edetate Disodium ~7.3 minutes
Total Run Time: 15 minutes
Posted: Tue Jul 11, 2006 7:45 pm
by jdlh199
Our HPLC method for measuring high concentration EDTA (5-1000 ug/mL EDTA) in solutions uses complexation with Iron. We add excess Iron to study samples, heat at 70oC for 30 mins, and then use HPLC to isolate Iron-EDTA. Conditions are:
Eluant: 20 ml/L TBAH adjusted to pH 3.5 with formic acid
Column: AquaSEP C18 100x3mm 5u
Sample: 30ul
Detection: UV @ 256nm
Retention time Fe-EDTA: ~2.5 mins
Run time: 6 mins
Posted: Tue Jul 11, 2006 8:24 pm
by rhaefe
We have an application note for iron EDTA (appnote #291):
Column: PRP-X100, 4.1x150mm (#79434)
Mobile Phase: 20mmol/L nitric acid
Flow Rate: 2mL/min
Inj.vol.: 10µL
UV at 254nm
Iron EDTA around 5min.
I can e-mail the application note in PDF format if needed.
rhaefele@hamiltoncompany.com
Posted: Wed Jul 12, 2006 1:04 pm
by Louise77
Hi rhaefe,
can i have a copy of the application note? Thanks.
cyloo1977@yahoo.com
Posted: Wed Jul 12, 2006 5:26 pm
by Bryan Evans
We have an application as well on our Unison UK-Phenyl column:
http://www.imtakt.com/TecInfo/TI261E.pdf
Posted: Sun Jul 16, 2006 3:38 pm
by Louise77
I am looking for this column, any recommendation?
spherical graphitized carbon for chromatography (5um)
with a surface area of 120 m2/g and pore size of 25 nm.
application: iron complex of edetic acid.
Posted: Wed Jul 19, 2006 10:03 pm
by Kostas Petritis
Actually these are the exact specifications from the porous graphitic carbon from Thermo (i.e. Hypercarb).
Have a look at:
http://www.interscience.nl/accessoires/ ... ercarb.pdf
There are other similar columns out there like the one form ZirCrhom but the specifications are different...