iron(III) EDTA complex

[Louise77]

Has anyone analysed iron(III) EDTA complex on HPLC? Any recommendation on the column? Zorbax?

[Albany-12303]

We test for EDTA by complexing it with copper (we put a small amount of CuSO4 in the dilutent and mobile phase). You can probably analyse Iron by putting some EDTA in the diluent/mobile phase

Something like this:

Column: Hamilton PRP-X100 ,10µ (250mm x 4.1mm)
Detector: 254nm UV
Inj Vol: 50µL
Flow: 1.0mL/min
Mobile Phase: 1M Sulfuric Acid 2.0mL
Phosphoric Acid, conc 2.0mL
Purified Water, USP 900mL
Methanol 100mL
0.1M Cupric Sulfate Solution 5.0mL
(Mix well and filter through a 0.45 micron membrane filter)

Retention Time of Edetate Disodium ~7.3 minutes
Total Run Time: 15 minutes

[jdlh199]

Our HPLC method for measuring high concentration EDTA (5-1000 ug/mL EDTA) in solutions uses complexation with Iron. We add excess Iron to study samples, heat at 70oC for 30 mins, and then use HPLC to isolate Iron-EDTA. Conditions are:

Eluant: 20 ml/L TBAH adjusted to pH 3.5 with formic acid
Column: AquaSEP C18 100x3mm 5u
Sample: 30ul
Detection: UV @ 256nm
Retention time Fe-EDTA: ~2.5 mins
Run time: 6 mins

[rhaefe]

We have an application note for iron EDTA (appnote #291):

Column: PRP-X100, 4.1x150mm (#79434)
Mobile Phase: 20mmol/L nitric acid
Flow Rate: 2mL/min
Inj.vol.: 10µL
UV at 254nm

Iron EDTA around 5min.

I can e-mail the application note in PDF format if needed.
rhaefele@hamiltoncompany.com

[Louise77]

Hi rhaefe,

can i have a copy of the application note? Thanks.

cyloo1977@yahoo.com

[Bryan Evans]

We have an application as well on our Unison UK-Phenyl column:

http://www.imtakt.com/TecInfo/TI261E.pdf

[Louise77]

I am looking for this column, any recommendation?

spherical graphitized carbon for chromatography (5um)
with a surface area of 120 m2/g and pore size of 25 nm.

application: iron complex of edetic acid.

[Kostas Petritis]

Actually these are the exact specifications from the porous graphitic carbon from Thermo (i.e. Hypercarb).

Have a look at: http://www.interscience.nl/accessoires/ ... ercarb.pdf

There are other similar columns out there like the one form ZirCrhom but the specifications are different...