peak purity by Refracive index detector in HPLC

[jchacko]

How do we determine the peak purity for stability indicatiing studies for a component that detected only by RI detector by HPLC??

[tom jupille]

You can't. Any type of "peak purity" requires a "sample specific" detector (one that responds to some property of the analyte). RI is a "bulk property" detector; it measures a property of the solvent which is altered by the presence of analyte.

[Rob Burgess]

If you have access to a LC-MS system you might be able to do peak purity on your RID detected component (assuming you know the mass of your analyte!)

[yogesh#14]

How do we determine the peak purity for stability indicatiing studies for a component that detected only by RI detector by HPLC??

Hi,

The RI detector does not have a SCAN feature as is available for the UV detector. This is because the RI detector functions on a different principle altogether. The only possibility of checking wheteher the method is stability indicating is that to attach a MS to the outlet of LC and check for any impurities or any coelutions..

Hope this should help you..

Regards,

Yogesh

[HW Mueller]

Or, if jchacko doesn´t have access to other machines: Set up a considerably different chromatography run the samples with the two methods, etc.